Thesis title: Site-specific Structure and Dynamics of Polyglutamine-containing Amyloid Fibrils and the Caveolin Scaffolding Domain by Magic Angle Spinning Solid-State NMR

Graduation date: Dec 2014

MBSB PhD Advisor: Dr. Patrick van der Wel (Dept. Structural Biology, University of Pittsburgh School of Medicine)

Research Interests:

Cody's PhD research was in the application of magic-angle spinning (MAS) solid-state NMR (ssNMR) techniques to study to structure and fibril-formation of amyloid proteins.  She specifically focused her research on the aggregation by polyglutamine-containing peptides and proteins, as implicated for instance in Huntington's Disease and related disorders. In addition, she used MAS NMR to study membrane-bound proteins and other protein aggregates.

Current location: postdoctoral researcher at Rutger's university, in the Baum lab

Education:

B.A., Chemistry, Washington & Jefferson College, 2008

Ph.D., Structural Biology & Molecular Biophysics, University of Pittsburgh, 2016

 Publications:

• Iuliucci, R.J., Hoop, C.L., Arif, A.M., Harper, J.K., Pugmire, R.J., and Grant, D.M. (2009) Redetermination of 1,4-dimethoxybenzene. (2009) Acta Crystallographica E. 65: o251
• Sivanandam VN, Jayaraman M, Hoop CL, Kodali R, Wetzel R, van der Wel PC. (2011) The aggregation-enhancing huntingtin N-terminus is helical in amyloid fibrils. J Am Chem Soc. 133(12):4558-66
• Li J, Hoop CL, Kodali R, Sivanandam VN, van der Wel PCA. (2011) Amyloid-like fibrils from a domain-swapping protein feature a parallel, in-register conformation without native-like interactions. J Biol Chem. 286(33):28988-95
• Hoop, CL, Sivanandam, VN, Kodali, R, Srnec, MN, and Van der Wel, PCA (2012) Structural Characterization of the Caveolin Scaffolding Domain in Association with Cholesterol-Rich Membranes. Biochemistry 51 (1), 90-99
• Mishra, R., Hoop, CL, Kodali, R., Sahoo, B, Van der Wel, PC, and Wetzel, R. (2012) Serine Phosphorylation Suppresses Huntingtin Amyloid Accumulation by Altering Protein Aggregation Properties J Mol Biol 424(1-2):1-14
• Hoop CL, Iuliucci RJ. 13C chemical-shift anisotropy of alkyl-substituted aromatic carbon in anthracene derivatives. Solid State Nucl Magn Reson. 2013 Jun;53:1-12.
• Kar K, Hoop CL, Drombosky KW, Baker MA, Kodali R, Arduini I, van der Wel PC, Horne WS, Wetzel R. (2013) β-hairpin-mediated nucleation of polyglutamine amyloid formation. J Mol Biol. 2013; 425(7):1183-97
• Hoop CL, Lin H, Kar K, Hou Z, Poirier MS, Wetzel R, van der Wel PC. (2014) Polyglutamine amyloid core boundaries and flanking domain dynamics in huntingtin fragment fibrils determined by solid-state NMR. Biochemistry. 53(42):6653-66
• Mandal, A., Hoop, C.L., DeLucia, M., Kodali, R., Kagan, V.E., Ahn, J., & van der Wel, P.C.A. (2015) Structural changes and pro-apoptotic peroxidase activity of cardiolipin-bound mitochondrial cytochrome c. Biophys J. in press.
• Hoop CL, Lin HK, Kar K, Magyarfalvi G, Lamley JM, Boatz JC, Mandal A, Lewandowski JR, Wetzel R, Van der Wel PC. Huntingtin exon 1 fibrils feature an interdigitated β-hairpin-based polyglutamine core. Proc Natl Acad Sci U S A. 2016;113(6):1546-51
• Kar K, Baker MA, Lengyel, GA, Hoop CL, Kodali R, Byeon, I-J, Horne WS, Van der Wel, PC, Wetzel R. Backbone engineering within a latent β-hairpin structure to design inhibitors of polyglutamine amyloid formation. J Mol Biol. 2017; in press