Thesis title: Biophysical Investigations of Aβ Aggregation Process
Graduation date: Dec 2014
PhD Advisor: Dr. Ronald Wetzel (Dept. Structural Biology, University of Pittsburgh School of Medicine)
Research interests:
Saketh's work in Dr. Wetzel’s lab involved studying structural biology of protein aggregation diseases and the mechanism of fibril formation. His work mainly focused on Amyloid-beta peptide molecules which are implicated in Alzheimer’s disease upon aggregation. His research included elucidation of the secondary structural elements of these aggregates using standard techniques like hydrogen-deuterium exchange along with mass spectrometry, FTIR and Transmission Electron Microscopy.
Current location: post-doctoral researcher at Case Western, in the Surewicz lab
Education:
B.Tech. Industrial Biotechnology, Anna University, India. 2006
Ph.D., Molecular Biophysics & Structural Biology, University of Pittsburgh, 2014
Publications:
- Kodali R, Williams AD, Chemuru S, Wetzel R. (2010) Abeta(1-40) forms five distinct amyloid structures whose beta-sheet contents and fibril stabilities are correlated. J Mol Biol. 2010;401(3):503-17
- Chemuru S, Kodali R, Wetzel R. Improved chemical synthesis of hydrophobic Aβ peptides using addition of C-terminal lysines later removed by carboxypeptidase B. Biopolymers. 2014;102(2):206-21
- Chemuru S, Kodali R, Wetzel R. (2016) C-Terminal Threonine Reduces Aβ43 Amyloidogenicity Compared with Aβ42. J Mol Biol. 428(2 Pt A):274-91
- Misra P, Kodali R, Chemuru S, Kar K, Wetzel R. (2016) Rapid α-oligomer formation mediated by the Aβ C terminus initiates an amyloid assembly pathway. Nat Commun. 7:12419
- Wetzel R, Chemuru S, Misra P, Kodali R, Mukherjee S, Kar K. An Aggregate Weight-Normalized Thioflavin-T Measurement Scale for Characterizing Polymorphic Amyloids and Assembly Intermediates. Methods Mol Biol. 2018;1777:121-144
