Lisa Clark

Research Interests:

My current research focuses on the parathyroid hormone receptor (PTHR), a G protein-coupled receptor critical to bone and kidney health. I am using computational and structural (e.g., NMR, EM) methods to understand the mechanisms of ligand binding, PTHR activation, and signaling. 

 

Education:

Biology BA, Truman State University, 2014

PhD Advisor: Dr. Jean-Pierre Vilardaga

Lab Address:

University of Pittsburgh School of Medicine
Dept. Pharmacology & Chemical Biology
W1301 Biomedical Science Tower, 200 Lothrop Street
Pittsburgh, PA 15261

email: ljc32 [at] pitt.edu

Publications:

  • Stevenson HP, Lin G, Barnes CO, Sutkeviciute I, Krzysiak T, Weiss SC, Reynolds S, Wu Y, Natarajan V, Makhov AM, Lawrence R, Lamm E, Clark L, Gardella TJ, Hogue BG, Ogata CM, Ahn J, Gronenborn AM, Conway JF, Vilardaga JP, Cohen AE, Calero G. (2016) Transmission electron microscopy for the evaluation and optimization of crystal growth. Acta Crystallogr D Struct Biol. 72(Pt 5):603-15
  • Xiao K, Zhao Y, Choi M, Liu H, Blanc A, Qian J, Xiao Y, Clark L, Li S. Revealing the architecture of protein complexes by an orthogonal approach combining HDXMS, CXMS, and disulfide Trapping. Nat Protoc. 2018 Jun;13(6):1403-1428.
  •  White AD, Fang F, Jean-Alphonse FG, Clark L et al. Ca2+ allostery in PTH-receptor signaling. Proc Natl Acad Sci U S A. 2019 Feb 19;116(8):3294-3299. 
  •  Zhao L-H, Ma S-S, Sutkeviciute I, Shen D-D, Zhou XE, de Waal PW, Li C-Y, Kang Y, Clark L et al. Structure and dynamics of parathyroid hormone receptor-1. Science. 2019 Apr 12;364(6436):148-153.

  • Ieva Sutkeviciute*, Lisa J. Clark*, Alex D. White, Thomas J. Gardella, and Jean-Pierre Vilardaga. Parathyroid Hormone Receptor: Structure, Allostery, and Signaling. Trends in Endocrinol & Metab. (2019) 30 (11):860-874 (Review). Special issue: Advancing topics in Endocrinology. doi:10.1016/j.tem.2019.07.011 *Contributed equally to this work.

  • Ieva Sutkeviciute, Lisa J. Clark, Jean-Pierre Vilardaga. Molecular mechanisms of parathyroid hormone type 1 receptor signaling. The Encyclopedia of Bone Biology (Chapt. 192), (Elsevier with Mone Zaidi, Editor-in-Chief) (2019) in press (Book Chapter). doi:10.1016/B978-0-12-801238-3.11264-4

  • Lisa J. Clark*, James Krieger*, Alex D. White, Vasyl Bondarenko, Saifei Lei, Fei Fang, Ji Young Lee, Pemra Doruker, Thore Böttke, Frederic Jean-Alphonse, Pei Tang, Thomas J. Gardella, Kunhong Xiao, Ieva Sutkeviciute, Irene Coin, Ivet Bahar, Jean-Pierre Vilardaga. Allosteric interactions in the parathyroid hormone class B GPCR–arrestin complex formation. Nat Chem Biol. (2020) *Contributed equally to this work. doi:10.1038/s41589-020-0567-0